A luminescence-based assay of UDP-sugar producing pyrophosphorylases

A luminescence-based assay of UDP-sugar producing pyrophosphorylases†

A common structural blueprint for plant UDP-sugar-producing pyrophosphorylases.

Plant pyrophosphorylases that are capable of producing UDP-sugars, key precursors for glycosylation reactions, include UDP-glucose pyrophosphorylases (A- and B-type), UDP-sugar pyrophosphorylase and UDP-N-acetylglucosamine pyrophosphorylase. Although not sharing significant homology at the amino acid sequence level, the proteins share a common structural blueprint. Their structures are characte...

Substrate Specificity and Inhibitor Sensitivity of Plant UDP-Sugar Producing Pyrophosphorylases

UDP-sugars are essential precursors for glycosylation reactions producing cell wall polysaccharides, sucrose, glycoproteins, glycolipids, etc. Primary mechanisms of UDP sugar formation involve the action of at least three distinct pyrophosphorylases using UTP and sugar-1-P as substrates. Here, substrate specificities of barley and Arabidopsis (two isozymes) UDP-glucose pyrophosphorylases (UGPas...

Identification and characterization of inhibitors of UDP-glucose and UDP-sugar pyrophosphorylases for in vivo studies.

UDP-sugars serve as ultimate precursors in hundreds of glycosylation reactions (e.g. for protein and lipid glycosylation, synthesis of sucrose, cell wall polysaccharides, etc.), underlying an important role of UDP-sugar-producing enzymes in cellular metabolism. However, genetic studies on mechanisms of UDP-sugar formation were frequently hampered by reproductive impairment of the resulting muta...

UDP-sugar pyrophosphorylase: a new old mechanism for sugar activation.

Abbreviations: aa, amino acid; AGPase, ADP-glc pyrophosphorylase; ara, arabinose; gal, galactose; galA, galacturonic acid; GALT, gal-1-P uridyltransferase; glc, glucose; glcA, glucuronic acid; man, mannose; PPi, pyrophosphate; SuSy, sucrose synthase; UAGPase, UDP-N-acetylglucosamine pyrophosphorylase; UGE, UDP-glc epimerase; UGPase, UDP-glc pyrophosphorylase; USPase, UDP-sugar pyrophosphorylase...